Fig. 6. HIF-1 hydroxylase expression in normoxia and responses to hypoxia, desferrioxamine (150 µM) or cobalt chloride (100 µM) treatment. U2OS total mRNA was reverse transcribed and subjected to quantitative PCR as detailed in Materials and Methods. Numbers above the bars indicate fold induction compared to normoxia. Results are given as means of four separate samples plus standard deviation.