Fig. 5. The CT and C2A of synaptotagmin VII are internalized by different pathways in PC12 cells. PC12 cells stably expressing either CT 7 (A,B) or C2A 7 (C,D) were transiently transfected with wild-type dynamin or dominant-negative dynamin K44E (A,C) or with control eps15 epsD32 or dominant-negative eps15 eps95/295 (B,D). The x-axis of the histograms shows the level of cell surface staining in each case. The curve for the wildtypes or controls are shown in black, and the curves for the dominant-negative mutants are shown in red. The mean of this value was taken and used to generate the calculated factor `cell surface fold increase' by dividing the mean surface staining in the dominant negative by the mean of the wildtype or control case (E).