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Fig. 6. The C2B of synaptotagmin VII is not internalized in PC12 cells. PC12 cells were generated that stably expressed CD4-C2B constructs for synaptotagmin I (residues 266-421, C2B 1) and synaptotagmin VII (residues 261-403, C2B 7). These cells were examined by the previously described radioactive internalization assay.