Fig. 2. Myosin Va is associated with SGs at the ultrastructural level. SGs from non-transfected PC12 cells were prepared by differential centrifugation (see Materials and Methods). SGs were then processed for immunoelectron microscopy in the absence (A) or presence (B) of anti-myosin Va, or with blocked anti-myosin Va (not shown), and protein A 10 nm immuno-gold (A,B). Bar, 500 nm. Unlabelled SGs (A, filled arrowheads) and labelled SGs (B, open arrowheads) are indicated. A quantification of the myosin Va immunolabelling of SGs is shown in C.