Fig. 3. Myosin Va cofractionates with SGs on sucrose gradients. A PNS of non-transfected PC12 cells was subjected to differential and then to equilibrium sucrose gradient (0.8-2 M sucrose) centrifugation. Equal aliquots of the final equilibrium gradient fractions (top, fraction 1) were analysed by western blotting for myosin Va and rSgII (A). The graph shows the intensity profiles of the two proteins (B). The membranes of fraction 9 of the gradient were pelleted, embedded in `Epon' and analysed by electron microscopy (C). Bar, 500 nm. The arrow indicates the magnified area shown at the bottom right.