Fig. 2. Direct interaction of LPP and
-actinin in a blot overlay.
Full-length LPP (right) as well as its N-terminal sequence (residues 1-109,
left) were expressed in bacteria as GST-fusion proteins and resolved on
polyacrylamide gels (0.1-2 µg/lane). After transfer to nitrocellulose, the
proteins were probed with radiolabeled
-actinin. GST (2 µg/lane) and
various protein standards were included as controls.