JCS -- Li et al. 116 (7): 1359 Figure IG5

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Fig. 5. Comparison of the subcellular distribution of GFP-tagged LPP and zyxin. H9C2 cells were transiently transfected with a full-length cDNA for LPP ligated into the expression vector pEGFP. Two days after transfection, the cells were fixed, permeabilized and stained with antibodies against ${alpha}$ -actinin and zyxin, respectively. The green panel shows direct fluorescence emitted from GFP, the red panel indirect immunofluorescence from antibodies against ${alpha}$ -actinin or zyxin. Bar, 20 µm.