Fig. 6. Recruitment of -actinin to LPP fusion proteins targeted to the surface of mitochondria. PtK2 cells were transiently transfected with GFP-fusion constructs encoding full-length LPP, full-length LPP lacking amino acids 41-57 (LPP), the N-terminal fragment of LPP (LPP61, residues 1-61) or a shorter fragment (LPP40, residues 1-40) as well as a mitochondrial anchor (M) as indicated. Two days after transfection, the cells were fixed, permeabilized and stained with antibodies against -actinin. The green panel shows direct fluorescence emitted from GFP, the red panel indirect immunofluorescence from antibodies against -actinin. Arrows point to large clusters of mitochondria. Bar, 20 µm.