Fig. 4. Nocodazole inhibits keratin particle movement (Movies 3 and 4). Panel A traces the course of one particle among the several visible keratin F particles in Movie 3, before treatment with nocodazole. Panels B-D are consecutive (20 second interval) frames, depicting the particle's movement (arrow). Panels E and F are two frames, taken several minutes apart, of Movie 4, after the addition of nocodazole (10 µg/ml). Nocodazole stops both F keratin particle movement and keratin filament wave-like motion (compare Movies 3 and 4). Both movies were compiled from 16 images taken at 20 second intervals. Z sections were taken with a 200 nm spacing. Images in A-F were obtained by projection of sections 4 and 5.