Fig. 1. Culture of mouse esophageal keratinocyte cells. (A) Phase contrast microscopy view of epithelial cells in primary culture. The dark area represents the explant tissue. (B) Phase contrast microscopy view of esophageal epithelioid cells at confluency (day 11). Scale bar, 10 µm. (C) Growth curve of long-term culture of normal mouse esophageal cells. Cell number is given as mean ± s.e.m. of three independent cultures.