Fig. 5. Kinetics of cyt c-GFP release following partial irradiation and effect of zVAD-fmk. Data show temporal distribution of cyt c-GFP expressing cells that exhibit release of cyt c-GFP, monitored at half hour intervals following irradiation. Cells were loaded with CMXRos and partially irradiated in the absence of zVAD-fmk (filled bars, n=31) or in the presence of zVAD-fmk (100 µM, pre-treated for 30 minutes; open bars, n=20). Each cell was monitored every 30 minutes up to 2.5 hours after irradiation and cyt c-GFP release was scored as percentage of total cells analysed. Asterisk (*) indicates cells that did not show cyt c-GFP release by 2.5 hours after irradiation; such cells may or may not release cyt c-GFP after this time. Hash symbol (#) indicates no significant difference in the proportion of cells showing cyt c-GFP release between the untreated group and zVAD-fmk treated group (P>0.3, chi-squared contingency test); all other comparisons on inspection are not significantly different.