Fig. 6. Bax is not recruited to non-irradiated mitochondria following partial irradiation. Cells were either subjected to partial irradiation (Irr) after labelling with CMXRos or treated with staurosporine (STS; 0.5 µM for 24 hours), or neither, before being fixed and stained with anti-Bax antibody. The photoirradiated cell was subjected to intensive laser scanning in its lower half (as oriented in this image; cf. Fig. 1) and fixed at 75 minutes after irradiation. Other cells partially irradiated were fixed for Bax staining at 30-150 minutes following irradiation. Bax translocation (Bax Trans) to mitochondria was visualised by confocal microscopy and scored as the percentage of the total number (n) of cells analysed in each treatment. Bars, 10 µm.