Fig. 4. Gene transfer of IB1/JIP-1 can prevent JNK activity in INS-1 cells. (A) JNK activity analysis in INS-1 revealed that cells incubated in presence of cytokines (IL-1ß, TNF- and IFN-) have a twofold increase in their JNK activity. The JNK content was evaluated by western blot and equal loading of substrate was confirmed by Coomassie-blue staining of the gel. (B) JNK activity analysis in INS-1 revealed that cells infected with Ad-asIB1 increased their JNK activity about twofold. By contrast, cells infected with ad-sIB1 showed a 50% decrease in their JNK activity.