Fig. 5. Quantitative comparison of the magnitude and direction of the movement of adhesions and nearby ECM landmarks. (A,B) Displacement data for two different cells undergoing extension. A significant correlation between adhesion and ECM displacement was found for every adhesion that was tracked (data from each adhesion are shown in a different color). However, the slope of the relationship varied from one adhesion to the next. (C) The pooled data for all five extensions analyzed. A highly significant correlation was found, but the magnitude of adhesion movement was larger than that of the ECM. (D) Data from a cell that underwent slow lamellipodial retraction. A high correlation between the magnitude of ECM and adhesion displacements (R=0.97, P<0.001), and the slope was much closer to 1.0. (E) Comparison of the direction of adhesion and ECM movements for all six experiments combined (five extensions and one retraction). A high correlation was demonstrated, and the slope was 1.0. (F) Since the adhesions tended to assume an oblong shape, we also measured the angle of the long axis of each adhesion (adhesion orientation). The adhesion orientation was highly correlated with the movement of the ECM.