Fig. 2. Western blot analysis of the p40/50 protein. (A) Comparison of p40/50 in C. tentans salivary glands and tissue culture cells (total extracts). Recombinant p40 served as control. An anti-p40/50 serum was used as primary antibody and an alkaline phosphatase-conjugated antibody as secondary antibody. (B) Demonstration of p40/50 in nuclear and cytoplasmic extracts from C. tentans tissue culture cells. The whole nuclear extract and 1/10 of the cytoplasmic extract were loaded onto SDS-polyacrylamide gels and blotted. The blots were either immunostained using an affinity-purified p40/50 antibody followed by an alkaline phosphatase-conjugated antibody (a), or stained with Coomassie blue (b). The positions of molecular mass standards are shown to the left in kilodaltons.