Fig. 3. Immunocytological localisation of p40/50 in C. tentans salivary gland cells. Semi-thin cryosections of salivary glands were prepared and immunostained with an affinity-purified antibody to p40/50 (A,A') or a pre-immune serum (B,B') as primary antibody and a gold-conjugated antibody as secondary antibody. The immunogold labelling was silver-enhanced and photographed both in bright-field (A,B) and phase-contrast (A',B') microscopy. n, nucleus; c, cytoplasm; nu, nucleolus. Bar, 20 µm.