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Fig. 9. Immunostaining by the anti-WSP-1 and anti-ARX-1 antibodies. (A) Anti-WSP-1 antibody conjugated with Alexa Fluor 546, (B) anti-ARX-1 antibody conjugated with Alexa Fluor 633, (C) expression of AJM-1::GFP and (D) the merged image are shown. WSP-1 is red, ARX-1 is blue and AJM-1 is green. The magnified image around the leading edge is shown in E, from left WSP-1, ARX-1, AJM-1 and the merged image. All images show the ventral view. The leading edge of the most anterior hypodermal cell is indicated with an arrowhead (A-E) and with a dotted line (E).