Fig. 3. Reduction of F-actin formation by arx-2(RNAi) and wsp-1(RNAi). Phalloidin staining of embryos carrying the jcIs1[ajm-1::GFP]. (A-D) Wild-type, (E-H) arx-2(RNAi) embryo during ventral enclosure and (I-L) wsp-1(RNAi) embryo at the terminal phenotype. Phalloidin staining (A,E,I), expression of AJM-1::GFP (B,F,J) and merged images (C,G,K) are shown. In merged images, phalloidin staining is red, and expression of AJM-1::GFP is green. Corresponding magnified images of phalloidin (left), AJM-1GFP (center), merged image (right) are also provided (D,H,L). The leading edge is indicated with arrows (A-L) and with dotted lines (D,H,L). In the wildtype, F-actin accumulation is observed at the leading edge (A,C,D). In arx-2(RNAi) and wsp-1(RNAi) embryos, F-actin accumulation is reduced at the leading edge (E,G,H,I,K,L).