Fig. 8. WSP-1 localizes at the periphery of cells. (A) Immunostaining of WSP-1 (a,d,g,j,n,r) and actin (b,e,h,k,o,s) in wild-type (a-c,j-m), as well as wsp-1(RNAi) (d-f,n-q) and arx-2(RNAi) (g-i,r-u) treated cells. Merged images are shown in c,f,i,l,p,t. Magnified images of migrating hypodermal cells are shown (m,q,u) for the anti-WSP-1 antibody (left), the anti-actin antibody (center) and for the merged images (right). WSP-1 localizes to the periphery of cells from early development to morphogenesis (a,j). Localization of WSP-1 is partially destabilized and observed in nuclei in arx-2(RNAi) (g,i,r,t,u) embryos. In j-u, anterior is left, posterior is right and ventral is bottom. The leading edges of the migrating hypodermal cells are indicated with arrows. (B) Western blotting of untreated and arx-1(RNAi)-treated embryo lysates with an anti-ARX-1 antibody (upper lane), anti-WSP-1 antibody (middle lane) and anti-actin antibody (bottom lane). In arx-1(RNAi) embryos, WSP-1 isoform1 is increased by four-fold, and the upper band that corresponds with WSP-1 isoform2 was detected.