Fig. 2. Detection of RhoA in membrane fractions from CD8 cells. (A) Fractions enriched in plasma membrane (low speed pellet, LS) or in intracellular vesicles (high speed pellet, HS) were prepared from CD8 cells in basal condition and after forskolin stimulation. Equal amount of protein (30 µg/lane) were subjected to western blotting analysis (see above) with anti-RhoA-specific antibody (1:500) and revealed with alkaline-phosphatase-conjugated anti-mouse IgG (1:5000). (B) Densitometric analysis (mean values±s.e., n=4) of the RhoA 22 kDa band. *P<0.001.