Fig. 3. (A) Detection of Rho-GDI in membrane fractions from CD8 cells. Membrane fractions enriched in plasma membrane (LS) or in intracellular vesicles (HS) were prepared from control and forskolin-stimulated CD8 cells. Equal amounts of protein (30 µg/lane) were separated by gel electrophoresis and immunoblotted with anti-Rho-GDI antibody (1:500). Results are representative of three independent experiments. (B) Densitometric profile (mean values±s.e., n=3) of the 29 kDa band. *P<0.001.