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Fig. 2. Zinc finger one is required for co-sedimentation with RNP in mammalian cells. Extracts obtained from transfected Cos7 cells were applied to 20-60% Nycodenz gradients. Western blots: (a) WT1 (+KTS); (b) WT1 (–KTS); (c) {Delta}R{Delta}F34; (d) {Delta}R{Delta}F234; (e) {Delta}R{Delta}F1234; (f) CT{Delta}F1 (+KTS); (g) native EGR1; (h) U5 snRNP associated splice factor p116; (i) WT1 pre-mRNA (RT-PCR).