Fig. 7. Localisation of ectopically expressed CT (WT1 C-terminus +KTS), CTF1, EGR1 and EGR1+F1 in the extrachromosomal nucleoli of stage IV oocytes. On expressing the T7-tagged proteins for 30 hours, immunostaining was seen to extend to the nucleoli. Although CT (A-C) and WT1 (not shown) located throughout the nucleolus, CTF1 was more localised to the DNA-containing fibrillar centres as seen in DAPI staining (E). Overexpressed EGR1 gave discrete localisation (G) at the DAPI-stained fibrillar centres (H). By contrast, EGR+F1 resembled CT (J-L). Bar, 10 µm.