Fig. 9. Nuclear extract from AC29 mouse mesothelioma cells was applied to anion exchange and a salt wash gradient applied. (A) Protein stain, showing flow through (FT) and increasing salt elutions. (B) Western blot showing the distribution of native WT1, the splice factors p116 and U2AF65, and EGR1. (C) Presence of RNA in fractions FT, 7, 9 and 11. Small nuclear RNA species, end-labelled by T4 RNA ligase, are shown above. The arrow points to U1 snRNA. WT1 pre-mRNA is shown below (RT-PCR).