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Fig. 6. (A) hPARP-3 or N-ter hPARP-3 overexpression leads to G1/S cell cycle arrest. FACS analysis on undamaged (left column) and MNU-treated (right column) HeLa cells expressed by GST, GST N-ter hPARP-3 or GST-hPARP-3. (B) hPARP-3 (d-f) or its N-terminal domain (a-c) target the GFP fusion protein to the centrosome, which is immunostained with an anti {gamma}-tubulin antibody (red). Bar, 10 µm.