Fig. 7. hPARP-3 overexpression does not prevent centrosome amplification induced by hydroxyurea (HU) in CHO cells. Cells were transfected for 48 hours to express full-length or N-ter hPARP-3 as GFP-fusion proteins and cultured in the presence (C-F) or absence (A-B) of HU. Transfected cells were identified by fluorescence microscopy, and the number of centrosomes quantified using the antibody anti-glutamylated tubulin Gt335 (red). (C,D) Non-tranfected cells. (E-G) Transfected cells expressing GFP-hPARP-3. (H) Histogram showing the mean number of centrosomes±s.d. counted in non-transfected cells or in cells expressing GFP alone, GFP-hPARP-3 or GFP-N-ter hPARP-3 following HU treatment. The arrows point to centrosome amplification. Bars, 10 µm.