Fig. 1. Co-localization of A. fumigatus conidia with endosomal markers in J774 cells. (A) J774 cells were infected with A. fumigatus conidia for 25 minutes (a) or 120 minutes (b) and then stained with an antibody to CD71 (a) or LAMP-1 (b). From left to right: fluorescence image showing the green channel (conidia); fluorescence image of the blue channel (red pseudocolor) (endosomes/lysosomes); and a merged overlay of both images. Bars, 10 µm. (B) The percentage of A. fumigatus phagosomes that contained either CD71 or LAMP-1 at 25, 45 or 120 minutes was scored by analysis of at least 100 internalized conidia in three separate experiments, each represented by a different symbol (circles, squares and diamonds).