Fig. 2. Co-localization of A. fumigatus conidia with endosomal markers in A549 cells. (A) A549 cells were infected with A. fumigatus conidia for 3 (a,b) or 24 hours (c) and then stained with an antibody to CD71 (a), CD63(b) or cathepsin D (c). From left to right: fluorescence image showing the green channel (conidia); fluorescence image of the blue channel (red pseudocolor) (endosomes/lysosomes); and a merged overlay of both images. Bars, 10 µm. (B) The percentage of A. fumigatus phagosomes that contained either CD71, CD63, LAMP-1 or cathepsin D at 3, 6 or 24 hours was scored by analysis of at least 100 internalized conidia in three separate experiments, each represented by a different symbol (circles, squares and diamonds).