Fig. 1. Expression of RyRs during limb development. (A) Western blot analysis of microsomal fractions prepared from hindlimbs from E11, 13, 15 and 17 reacted with the antisera specific to type 1 (top) or type 3 (bottom) RyRs, as indicated. (B) Western blot analysis of microsomal fractions prepared from HEK293 cells transfected with RyR1 or RyR3 expression vectors and incubated with the anti-RyR1 or RyR3 antibody, as indicated.