Fig. 2. Immunolocalization of RyRs in E13 limb muscle. Double immunofluorescence analysis of hind limb cryosections from E13 embryos; the same section was incubated with anti-RyR1 antiserum (A) and anti-MyHC antibody MF20 (B), or with anti-RyR3 antiserum (D) and MF20 (E). Phase contrast micrographs are also shown for each section (C,F). Bar, 10 µm.