Fig. 9. Agents that inhibit anti-ICAM-1 immunobead uptake disrupt actin rearrangements induced by immunobeads. HUVEC were pretreated with 10 µM latrunculin A (A), 10 µM radicicol (B), 10 µM Y-27632 (C) or 3 mM amiloride (D), incubated with anti-ICAM-1 immunobeads for 15 minutes then fixed and stained for filamentous actin using rhodamine phalloidin. Each of these agents that inhibit uptake of anti-ICAM-1 immunobeads also inhibited actin stress-fiber formation. Bar, 10 µm.