Fig. 4. VCX1 plays an important role in Ca2+ homeostasis in the
pmc1
and pmr1
/pmc1
strains.
(A) Total cellular Ca2+ levels measured with flame photometry. (B)
Cytosolic free Ca2+ levels measured with an aequorin
Ca2+ reporter system. The arrow represents the addition of 100 mM
CaCl2 (see Materials and Methods for further details).