Fig. 1. Specificity of antibodies to Eg7G, XCAP-E1, TopoII and ß-tubulin in XL2 cell extract. Proteins in X. laevis XL2 cell lysate (2x10⁵ cells) were separated onto 7% SDS-polyacrylamide gel, transferred onto nitrocellulose membranes and immunodetected with mixture of purified Eg7G and monoclonal antibodies against ß-tubulin (lane1) or XCAP-E1 antibodies (lane 2) or monoclonal anti-topoII antibodies (lane 3).