Fig. 2. Western blot analysis of the quantity of pEg7, topoII and XCAP-E in the synchronized cells of different stages of cell cycle. (A) After electrophoresis and transfer onto nitrocellulose, the membrane was cut, the upper part was incubated with antibodies to topoII, the second part with a mixture of pEg7 and the third part with monoclonal antibodies against ß-tubulin and the last part with monoclonal antibodies against pEg2 (clone 1C1). Immunocomplexes were revealed using an enhanced chemoluminescence system. (B) Relative quantities of protein during the cell cycle (protein:ß-tubulin ratio, with the ratio in G1 equal to 1). The level of cycle-dependent protein kinase pEg2 increased from 1 at G1 to 15.44±3.21 at M phase; the quantity of topoII was maximal in G2 phase (3.61±1.22) and practically the same in mitosis (3.52±1.04). The quantity of pEg7 and XCAP-E increased from 1 at G1 to 1.48±0.09 and 1.48±0.04 times at M phase, respectively. Average data from four measurements from two experiments are presented. Quantitative analysis was performed using Image Quant computer program.