Fig. 7. Immunolocalization of XCAP-E and B23 in control cells and after actinomycin treatment. XL2 cells were incubated for 6 hours in the medium with 5 µg/ml actinomycin D (E-H) or without the drug (A-D). After fixation, cells were processed for immunofluorescence staining with polyclonal anti-XCAP-E1 (B,F) and monoclonal anti-B23 (C,G) antibodies. Cells were stained for DNA visualization with DAPI (A,E). Triple DAPI/XCAP-E/B23 labeling is shown (D,H). Bar, 5 µm.