Fig. 2. Structure of Apg16L. (A) Amino acid sequence of Apg16L. Underlined and dotted-underlined residues correspond to peptide sequences obtained by MS analysis of p63 and p71, respectively. Boxes indicate the peptides encoded by exons 8 and 9, which are deleted in spliced isoforms. Sequences outlined in black are WD repeats. (B) Amino acid sequence of mouse Apg16L was aligned with that of S. cerevisiae Apg16 using the BLAST2 program (http://www.ncbi.nlm.nih.gov/blast/bl2seq/bl2.html). Identical amino acids are shown with a line between them (|), while similar amino acids are indicated with dots (:). (C) Structural comparison of putative Apg16L homologues. The coiled-coil regions are indicated as gray boxes [analyzed by Multicoil program (Wolf et al., 1997), http://multicoil.lcs.mit.edu/cgi-bin/multicoil] and the WD repeats are shown as black boxes (analyzed by PSA Sequence analysis, http://bmerc-www.bu.edu/psa/request.htm). At, Arabidopsis thaliana (AB024031); Ce, Caenorhabditis elegance (U53340, U23449); Dd, Dictyostelium discoideum (AF019236); Dm, Drosophila melanogaster (AY058742); Hs, Homo sapiens (AK027854, AK024453); Mm, Mus musculus; Os, Oryza sativa (AC087852); Pp, Pichia pastoris (Mukaiyama et al., 2002) (Y. Sakai, personal communication); Sc, Saccharomyces cerevisiae. The amino acid sequences of Dm Apg16L and Ce Apg16L2, hypothesized from the genomic sequences, might be incomplete, lacking N-terminal sequences.