Fig. 6. Apg16L co-localizes completely with Apg5 and in part with LC3. ES cells stably co-expressing YFP-LC3 and CFP-Apg5 (A; clone F1-3), CFP-Apg5 and YFP-Apg16L (B; clone Y63D-3), and YFP-LC3 and CFP-Apg16L (C; clone B3-1-5) were cultured in Hanks' solution for 2 hours. Living cells were directly observed with a DeltaVision microscope system. ES cells grow as colonies; 4-7 cells are shown in each panel. Bars, 2 µm.