JCS -- Mizushima et al. 116 (9): 1679 Figure IG7

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Fig. 7. GFP-Apg16L is present on isolation membranes. ES cells stably expressing GFP-Apg16L were cultured in Hanks' solution for 2 hours and then fixed with 4% paraformaldehyde. The localization of GFP-Apg16L was examined by silver-enhanced immunogold electron microscopy using an anti-GFP antibody. (A,B) Isolation membranes at very early stages. (C) Cup-shaped isolation membrane. (D) Autophagosome. Bar, 1 µm.