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Fig. 8. Membrane association of Apg16L depends on Apg5 but not on Apg5 conjugation with Apg12. APG5-/- ES cells stably expressing GFP-Apg5 (GFP24) (A) or GFP-Apg5K130R (GKR-1) (B), wild-type ES cells (C) and APG5-/- ES cells (D) were cultured in Hanks' solution for 2 hours. The cells were fixed, permeabilized and subjected to immunofluorescence confocal microscopy using an antiserum against Apg16L (p63C-2) and Cy5-conjugated goat anti-rabbit IgG secondary antibody. GFP-Apg5(KR) labeling, Apg16L staining, merged, and differential interference contrast (DIC) images are shown. Bars, 10 µm.