Fig. 5. eng1p protein is synthesized periodically during the cell cycle. cdc25-22 mutant cells carrying the eng1-GFP fusion allele integrated in the chromosome were arrested by incubation at the restrictive temperature (37°C) for 4 hours and then transferred to the permissive temperature (25°C) to monitor the localization of the fusion protein. Numbers indicate minutes after transfer to the permissive temperature.