Fig. 6. eng1p localizes to the septum. (A) Wild-type cells containing the eng1-GFP fusion allele were grown to early-log phase and stained with Calcofluor before microscopic observation. Calcofluor fluorescence (CF), GFP fluorescence and the overlay of both images (Merge) are shown. (B) Three-dimensional reconstruction of eng1p localization. Cells containing the eng1-GFP fusion allele were observed under a confocal microscope and z-sections of 0.3 µm were taken. The image was reconstructed using LSM510 software.