Fig. 8. ace2⁺ is required for cell separation. (A) Expression of eng1⁺ and act1⁺ genes in wild-type (lane 1), ace2-null mutants (cells lacking the SPAC6G10.12C ORF, lane 2), or cells containing a deletion of SPAC4G8.13c (lane 3). (B) Morphology of the mutant cells grown in rich medium to early-log phase. Wild-type (1) and mutants lacking SPAC4G8.13c (2) show a normal morphology, while ace2 mutants (3) have a clear mycelial and branched phenotype, indicating severe cell separation defects.