Fig. 1. Localization of EAST protein in dividing cells. The distribution of EAST in cells of mitotic domains was revealed by triple labeling post-syncytial embryos with anti-EAST antibody (green), anti-tubulin antibody (white) and DNA dye (red). Throughout interphase (A,H) and prophase (B), EAST shows nuclear localization. At prometaphase (C), metaphase (D) and early anaphase (E), EAST remains in the center of the cell associated with the remnant of the interphase nucleus. EAST labeling decays at late anaphase (F) and is barely detectable at telophase (G). Intense staining is again seen in early interphase (H).