Fig. 4. Loss of maternal east results in pleiotropic mitotic and developmental defects. Loss of maternal east expression in east^hop-1 germline clones coupled with zygotic expression from one wt copy of east can lead to a variety of phenotypes, ranging from fertile female escapers without discernible morphological abnormalities (A), to adults showing a loss of various morphological structures (B), like tergites on the abdomen (arrowheads) or appendages (arrow). Lethality, along with variable developmental defects, was also observed during embryonic development. In late control embryos (C), the in vivo GFP marker of the balancer FM7i-pAct—GFP labels the midgut. (D) Loss of maternal east can cause the GFP expression to appear in the anterior regions of the embryo. (E,F) Nuclei of the syncytial blastoderm stage in wt (E) and east(mat) (F) embryos stained with anti-histone antibody. Loss of maternal east can lead to the elimination of nuclei from the surface (F). (G,H) Syncytial blastoderm embryos stained with anti-tubulin (green) and anti-histone (red) antibodies. (G) Polyploid nuclei at metaphase and (H) orphan centrosomes in anaphase (arrowheads) without daughter nuclei (arrows) indicate mitotic errors. Bars, 100 µm (C-F) and 10 µm (G-J).