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Fig. 3. FHOD1 interacts with F-actin and G-actin. (A) NIH-3T3 cells were transiently transfected with plasmids encoding full-length HA-FHOD1 (1-1165), HA-FHOD1 {Delta}C (1-1010) or HA-FHOD1 (1-421). FHOD1 expression was detected by in situ staining with anti-HA followed by FITC-conjugated secondary antibodies. Cells were countered stained with phalloidin-rhodamine to detect F-actin. Images were collected by confocal microscopy. (B) HEK293T cells were transiently transfected with expression vectors for the indicated HA-FHOD1 proteins. Triton X-100 soluble cellular proteins were immunoprecipitated with anti-HA or anti-actin antibodies. Proteins were detected by immunoblotting with anti-HA or anti-actin antibodies and enhanced chemiluminescence.