Fig. 5. FHOD1 expression does not affect cell adhesion. (A) FHOD1 expression does not significantly affect WM35 cell adhesion to type-I collagen. WM35 cells were allowed to adhere to plates coated with the indicated concentrations of type-I collagen for 20 minutes. Adherent cells were quantified with a cell viability assay. The number of adherent cells was normalized to a standard curve generated by plating increasing numbers of cells. (B) FHOD1 expression does not significantly affect NIH-3T3 cell adhesion to fibronectin. NIH-3T3 cells were allowed to adhere to plates coated with the increasing concentrations of fibronectin for 20 minutes. The number of adherent cells was determined as described in A.