Fig. 7. FHOD1 expression does not affect integrin expression or use. (A) FHOD1 does not significantly alter integrin expression on WM35 cells. Stable WM35 cells were incubated with PE-conjugated anti-integrin antibodies and analyzed by flow cytometry. The mean PE fluorescence intensity of eGFP-positive cells was determined with CellQuest software. (B) FHOD1 expression does not alter integrin usage during cell adhesion. Cells were preincubated for 20 minutes with 2.5 µg ml^-1 of the isotype-matched control or the indicated -integrin mAb, or with 1 µg ml^-1 of anti-ß₁ mAb. The amount of cell adhesion to plates coated with 15 µg ml^-1 type-I collagen within 20 minutes was determined with a cell viability assay. Values for each cell line were normalized to the control cells incubated with the isotype-matched mAb. (C) FHOD1 does not alter integrin usage during cell migration. WM35-MSCV control (left) or FHOD1-expressing (right) cells were preincubated for 20 minutes with 2.5 µg ml^-1 of the isotype-matched control or the indicated -integrin mAb or with 1 µg ml^-1 of anti-ß1 mAb. Cell migration during a 4.5 hour period was measured in a modified Boyden chamber assay.