Fig. 3. Inhibition of the catalytic subunit of telomerase by RNAi. (a) Construct used for the inhibition of telomerase. A map of the gene encoding the catalytic subunit of telomerase (p123) is shown. The 573 bp construct cloned into p4440 and the primers used are indicated. Motifs CP, T, 1, 2, A, B', C, D and E are shown (Bryan et al., 1998). (b) Before (left) and after (right) inhibition. (c) Distribution of p43 in the macronucleus after inhibition of telomerase expression (left), electroeluted macronuclei after inhibition of telomerase expression stained with an anti-p43 antibody (right). Bar, 10 µm.