Fig. 4. Binding of the p43-telomerase complex to the nuclear matrix. (a) In situ staining of macronuclear halos with DAPI (left) and an anti-p43 antibody (right). (b) Macronuclear halos stained with DAPI (left) and FISH analysis of the telomerase RNA subunit (right). p43 staining after electroelution (c) and after RNAse treatment followed by electroelution (d). Macronuclear halos before (e) and after inhibition of p43 expression by RNAi (f) stained with DAPI (left) and FISH analysis of the telomerase RNA subunit (right). Bar 10 µm.