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Fig. 5. Cleavage of MT1-MMP pro-domain by furin in isolated glomerular membranes. (A,B) Total membrane fractions of isolated rat glomeruli, untreated (lane 1) or digested with recombinant soluble furin (lane 2), were resolved by 7.5% SDS-PAGE and analysed by western blotting. (A) The 63 kDa band revealing proMT1-MMP in native membranes (lane 1) disappears upon addition of soluble recombinant furin (lane 2), indicating that cleavage of the pro-domain has occurred. (B) In order to assess the integrity of other glomerular membrane proteins, the same blot as in A was stripped and reprobed for the ß3 integrin subunit. The appearance of a ~110 kDa band in both lanes indicates the specificity of the limited proteolysis of proMT1-MMP by furin.